Please use this identifier to cite or link to this item:
https://doi.org/10.1128/spectrum.00849-22
DC Field | Value | |
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dc.title | Mutual Exclusion of <i>Methanobrevibacter</i> Species in the Human Gut Microbiota Facilitates Directed Cultivation of a <i>Candidatus</i> Methanobrevibacter Intestini Representative | |
dc.contributor.author | Low, Adrian | |
dc.contributor.author | Lee, Jolie Kar Yi | |
dc.contributor.author | Gounot, Jean-Sebastien | |
dc.contributor.author | Ravikrishnan, Aarthi | |
dc.contributor.author | Ding, Yichen | |
dc.contributor.author | Saw, Woei-Yuh | |
dc.contributor.author | Tan, Linda Wei Lin | |
dc.contributor.author | Moong, Don Kyin Nwe | |
dc.contributor.author | Teo, Yik Ying | |
dc.contributor.author | Nagarajan, Niranjan | |
dc.contributor.author | Seedorf, Henning | |
dc.date.accessioned | 2024-04-01T09:17:01Z | |
dc.date.available | 2024-04-01T09:17:01Z | |
dc.date.issued | 2022-08-01 | |
dc.identifier.citation | Low, Adrian, Lee, Jolie Kar Yi, Gounot, Jean-Sebastien, Ravikrishnan, Aarthi, Ding, Yichen, Saw, Woei-Yuh, Tan, Linda Wei Lin, Moong, Don Kyin Nwe, Teo, Yik Ying, Nagarajan, Niranjan, Seedorf, Henning (2022-08-01). Mutual Exclusion of <i>Methanobrevibacter</i> Species in the Human Gut Microbiota Facilitates Directed Cultivation of a <i>Candidatus</i> Methanobrevibacter Intestini Representative. MICROBIOLOGY SPECTRUM 10 (4). ScholarBank@NUS Repository. https://doi.org/10.1128/spectrum.00849-22 | |
dc.identifier.issn | 2165-0497 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/247681 | |
dc.description.abstract | Methanogenic Archaea (methanogens) are a phylogenetically diverse group of microorganisms and are considered to be the most abundant archaeal representatives in the human gut. However, the gut methanogen diversity of human populations in many global regions remains poorly investigated. Here, we report the abundance and diversity of gut methanogenic Archaea in a multi-ethnic cohort of healthy Singaporeans by using a concerted approach of metagenomic sequencing, 16S rRNA gene amplicon sequencing, and quantitative PCR. Our results indicate a mutual exclusion of Methanobrevibacter species, i.e., the highly prevalent Methanobrevibacter smithii and the less prevalent Candidatus Methanobrevibacter intestini in more than 80% of the samples when using an amplicon sequencing-based approach. Leveraging on this finding, we were able to select a fecal sample to isolate a representative strain, TLL-48-HuF1, for Candidatus Methanobrevibacter intestini. The analyzed physiological parameters of M. smithii DSM 861T and strain TLL-48-HuF1 suggest high similarity of the two species. Comparative genome analysis and the mutual exclusion of the Methanobrevibacter species indicate potentially different niche adaptation strategies in the human host, which may support the designation of Candidatus M. intestini as a novel species. | |
dc.language.iso | en | |
dc.publisher | AMER SOC MICROBIOLOGY | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Life Sciences & Biomedicine | |
dc.subject | Microbiology | |
dc.subject | Archaea | |
dc.subject | human gut microbiota | |
dc.subject | Methanobrevibacter | |
dc.subject | methanogens | |
dc.subject | METHANOGENIC ARCHAEA | |
dc.subject | GENOME | |
dc.subject | DIVERSITY | |
dc.subject | QUALITY | |
dc.type | Article | |
dc.date.updated | 2024-04-01T06:35:01Z | |
dc.contributor.department | MEDICINE | |
dc.contributor.department | LIFE SCIENCES INSTITUTE | |
dc.contributor.department | DEAN'S OFFICE (SSH SCH OF PUBLIC HEALTH) | |
dc.contributor.department | SAW SWEE HOCK SCHOOL OF PUBLIC HEALTH | |
dc.contributor.department | DEPARTMENT OF COMPUTER SCIENCE | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1128/spectrum.00849-22 | |
dc.description.sourcetitle | MICROBIOLOGY SPECTRUM | |
dc.description.volume | 10 | |
dc.description.issue | 4 | |
dc.published.state | Accepted | |
Appears in Collections: | Staff Publications Elements |
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