Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bbrc.2005.12.133
DC Field | Value | |
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dc.title | Suppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein | |
dc.contributor.author | Lu, L. | |
dc.contributor.author | Ho, Y. | |
dc.contributor.author | Kwang, J. | |
dc.date.accessioned | 2011-07-26T06:54:06Z | |
dc.date.available | 2011-07-26T06:54:06Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Lu, L., Ho, Y., Kwang, J. (2006). Suppression of porcine arterivirus replication by baculovirus-delivered shRNA targeting nucleoprotein. Biochemical and Biophysical Research Communications 340 (4) : 1178-1183. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2005.12.133 | |
dc.identifier.issn | 0006291X | |
dc.identifier.issn | 10902104 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/24751 | |
dc.description.abstract | The ability to synthesize shRNAs from DNA templates driven by RNA polymerase III promoters has made it possible to apply virus-derived vectors as delivery vehicles for double-strand RNA-mediated interference. Baculovirus emerges as a promising vector for in vivo gene therapy most recently. To investigate its potential as a delivery vector for anti-virus shRNA targeting arterivirus porcine reproductive and respiratory syndrome virus (PRRSV), we constructed recombinant baculovirus vectors bearing a shRNA-synthesizing cassette driven by U6 promoter with enhanced transduction efficiency by displaying vesicular stomatitis virus glycoprotein on viral envelope. Transduction of Marc145 cells with a recombinant baculovirus delivering egfp gene-specific shRNA dramatically suppressed the expression of EGFP in this cell line; and transduction of Marc145 cells with a baculovirus delivering shRNA specific for the C-terminal nucleoprotein coding region of PRRSV genome resulted in inhibition of viral replication. Our data highlight the recombinant baculovirus as an alternative vehicle for anti-virus shRNA delivery. © 2005 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2005.12.133 | |
dc.source | Scopus | |
dc.subject | Baculovirus | |
dc.subject | Marc145 | |
dc.subject | PRRSV | |
dc.subject | RNAi | |
dc.subject | SF9 | |
dc.subject | shRNA | |
dc.subject | VSV G | |
dc.type | Article | |
dc.contributor.department | MICROBIOLOGY | |
dc.description.doi | 10.1016/j.bbrc.2005.12.133 | |
dc.description.sourcetitle | Biochemical and Biophysical Research Communications | |
dc.description.volume | 340 | |
dc.description.issue | 4 | |
dc.description.page | 1178-1183 | |
dc.identifier.isiut | 000235041500023 | |
Appears in Collections: | Staff Publications |
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