Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.femsle.2005.06.024
Title: Differences in biochemical properties of the Plasmodial falcipain-2 and berghepain-2 orthologues: Implications for in vivo screens of inhibitors
Authors: Chan, C.
Goh, L.L.
Sim, T.-S. 
Keywords: Animal model
Berghepain
Falcipain
Issue Date: 2005
Source: Chan, C., Goh, L.L., Sim, T.-S. (2005). Differences in biochemical properties of the Plasmodial falcipain-2 and berghepain-2 orthologues: Implications for in vivo screens of inhibitors. FEMS Microbiology Letters 249 (2) : 315-321. ScholarBank@NUS Repository. https://doi.org/10.1016/j.femsle.2005.06.024
Abstract: Falcipain-2A, the cysteine protease of Plasmodium falciparum has been proposed as a good drug target. This study evaluated the suitability of Plasmodium berghei as the animal model and reports the first functional expression and characterization of the falcipain-2A orthologue, berghepain-2. Comparative studies revealed that the orthologues exhibited different biochemical properties. Berghepain-2 demonstrated optimal activity at a narrower pH optima of 5.5-6 and a lack of preference for substrates with leucine at position 2. Mutagenesis studies revealed roles for residues Val63 and Arg230 of berghepain-2 in contributing to its distinctive biochemical properties. This warrants re-evaluation of employing P. berghei as the murine model for the in vivo screening of falcipain-2A inhibitors. More importantly, these findings stress the underlying importance of establishing the functionality of relevant genes of P. falciparum with concomitant relevance to its murine counterpart prior to its use as the animal model for the screening of potential antimalarials. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Source Title: FEMS Microbiology Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/24731
ISSN: 03781097
DOI: 10.1016/j.femsle.2005.06.024
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

8
checked on Dec 14, 2017

Page view(s)

144
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.