Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.resmic.2006.10.001
Title: Alterations in rdxA and frxA genes and their upstream regions in metronidazole-resistant Helicobacter pylori isolates
Authors: Han, F.
Yan, Z.
Yan, X.
Liu, S.
Ho, B. 
Keywords: frxA gene
Helicobacter pylori
Metronidazole
Mutation
rdxA gene
Resistance
Issue Date: 2007
Source: Han, F., Yan, Z., Yan, X., Liu, S., Ho, B. (2007). Alterations in rdxA and frxA genes and their upstream regions in metronidazole-resistant Helicobacter pylori isolates. Research in Microbiology 158 (1) : 38-44. ScholarBank@NUS Repository. https://doi.org/10.1016/j.resmic.2006.10.001
Abstract: Metronidazole resistance among Helicobacter pylori strains has been related to alterations in gene products having metronidazole nitroreductase activities. RdxA and FrxA proteins are the two major contributing factors. In this investigation, the rdxA and frxA genes and their upstream regions were analyzed in 19 H. pylori isolates, 8 of which were metronidazole-sensitive (MIC ≤ 8 μg/mL) and 11 of which were metronidazole-resistant (MIC ≥ 8 μg/mL), as determined by the E-test. Among the metronidazole-resistant isolates, three contained both RdxA and FrxA proteins with premature truncation caused by gene nonsense mutations or frameshift mutations, while three contained only stop mutations in FrxA and two only in RdxA. Substitutions of amino acids occurred in other RdxA (5/6) and FrxA (4/5) proteins from metronidazole resistant isolates as compared with those from metronidazole-sensitive ones. All metronidazole-resistant isolates had alterations in RdxA and/or FrxA proteins. Moreover, the upstream regions (-1 to -35) of rdxA and frxA genes in some metronidazole-resistant isolates varied by nucleotide insertion and/or deletion or substitution. The patterns of variation in both genes and their upstream regions were highly diversified. Alterations in rdxA and frxA genes and their upstream regions may be involved in the development of metronidazole resistance in H. pylori. © 2006 Elsevier Masson SAS. All rights reserved.
Source Title: Research in Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/24718
ISSN: 09232508
17697123
DOI: 10.1016/j.resmic.2006.10.001
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