Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jmb.2008.03.069
Title: Nhp6p and Med3p Regulate Gene Expression by Controlling the Local Subunit Composition of RNA Polymerase II
Authors: Xue, X.
Lehming, N. 
Keywords: chromatin
protein interaction
recruitment
split ubiquitin
transcription
Issue Date: 2008
Source: Xue, X., Lehming, N. (2008). Nhp6p and Med3p Regulate Gene Expression by Controlling the Local Subunit Composition of RNA Polymerase II. Journal of Molecular Biology 379 (2) : 212-230. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jmb.2008.03.069
Abstract: Nhp6p is an architectural Saccharomyces cerevisiae non-histone chromosomal protein that bends DNA and plays an important role in transcription and genome stability. We used the split-ubiquitin system to isolate proteins that interact with Nhp6p in vivo, and we confirmed 11 of these protein-protein interactions with glutathione S-transferase pull-down experiments in vitro. Most of the Nhp6p-interacting proteins are involved in transcription and DNA repair. We utilized the ZDS1, PUR5 and UME6 genes, which are repressed by Nhp6p and its interacting partners Rpb4p and Med3p, to study the chromosomal localization of these three proteins in wild-type and gene deletion strains. Nhp6p, Med3p and Rpb4p were found at the promoters of ZDS1, PUR5 and UME6, indicating that the repressing effects the three proteins had on the expression of these three genes had been direct ones. We also found that Med3p inhibited promoter clearance of RNA polymerase II, which contained the dissociable subunit Rpb4p, while Nhp6p recruited Rpb4p to the basal promoters of ZDS1, PUR5 and UME6. Our results further suggest that Rpb4p inhibits transcription initiation but stimulates transcription elongation and that Nhp6p and Med3p regulate gene expression by controlling the local subunit composition of RNA polymerase II. © 2008 Elsevier Ltd. All rights reserved.
Source Title: Journal of Molecular Biology
URI: http://scholarbank.nus.edu.sg/handle/10635/24658
ISSN: 00222836
DOI: 10.1016/j.jmb.2008.03.069
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

6
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

6
checked on Nov 22, 2017

Page view(s)

182
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.