Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2008.07.078
Title: AFM indentation study of breast cancer cells
Authors: Li, Q.S.
Lim, C.T.
Lee, G.Y.H.
Ong, C.N. 
Keywords: Actin cytoskeleton
Atomic force microscopy
Breast cancer disease
Cell mechanics
Confocal microscopy
Elasticity
Hertz's contact model
Stress fiber
Issue Date: 2008
Source: Li, Q.S., Lim, C.T., Lee, G.Y.H., Ong, C.N. (2008). AFM indentation study of breast cancer cells. Biochemical and Biophysical Research Communications 374 (4) : 609-613. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2008.07.078
Abstract: Mechanical properties of individual living cells are known to be closely related to the health and function of the human body. Here, atomic force microscopy (AFM) indentation using a micro-sized spherical probe was carried out to characterize the elasticity of benign (MCF-10A) and cancerous (MCF-7) human breast epithelial cells. AFM imaging and confocal fluorescence imaging were also used to investigate their corresponding sub-membrane cytoskeletal structures. Malignant (MCF-7) breast cells were found to have an apparent Young's modulus significantly lower (1.4-1.8 times) than that of their non-malignant (MCF-10A) counterparts at physiological temperature (37 °C), and their apparent Young's modulus increase with loading rate. Both confocal and AFM images showed a significant difference in the organization of their sub-membrane actin structures which directly contribute to their difference in cell elasticity. This change may have facilitated easy migration and invasion of malignant cells during metastasis. © 2008 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/24463
ISSN: 0006291X
10902104
DOI: 10.1016/j.bbrc.2008.07.078
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

391
checked on Dec 7, 2017

WEB OF SCIENCETM
Citations

364
checked on Nov 23, 2017

Page view(s)

300
checked on Dec 11, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.