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Title: AFM indentation study of breast cancer cells
Authors: Li, Q.S.
Lim, C.T.
Lee, G.Y.H.
Ong, C.N. 
Keywords: Actin cytoskeleton
Atomic force microscopy
Breast cancer disease
Cell mechanics
Confocal microscopy
Hertz's contact model
Stress fiber
Issue Date: 2008
Source: Li, Q.S., Lim, C.T., Lee, G.Y.H., Ong, C.N. (2008). AFM indentation study of breast cancer cells. Biochemical and Biophysical Research Communications 374 (4) : 609-613. ScholarBank@NUS Repository.
Abstract: Mechanical properties of individual living cells are known to be closely related to the health and function of the human body. Here, atomic force microscopy (AFM) indentation using a micro-sized spherical probe was carried out to characterize the elasticity of benign (MCF-10A) and cancerous (MCF-7) human breast epithelial cells. AFM imaging and confocal fluorescence imaging were also used to investigate their corresponding sub-membrane cytoskeletal structures. Malignant (MCF-7) breast cells were found to have an apparent Young's modulus significantly lower (1.4-1.8 times) than that of their non-malignant (MCF-10A) counterparts at physiological temperature (37 °C), and their apparent Young's modulus increase with loading rate. Both confocal and AFM images showed a significant difference in the organization of their sub-membrane actin structures which directly contribute to their difference in cell elasticity. This change may have facilitated easy migration and invasion of malignant cells during metastasis. © 2008 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2008.07.078
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