Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.neulet.2009.03.038
Title: Apolipoprotein D modulates F2-isoprostane and 7-ketocholesterol formation and has a neuroprotective effect on organotypic hippocampal cultures after kainate-induced excitotoxic injury
Authors: He, X.
Jittiwat, J. 
Kim, J.-H.
Ong, W.-Y. 
Jenner, A.M. 
Farooqui, A.A.
Patel, S.C.
Keywords: 7-Ketocholesterol
Apolipoprotein D
Excitotoxicity
Lipid peroxidation
Oxidative stress
Oxysterols
Issue Date: 2009
Source: He, X., Jittiwat, J., Kim, J.-H., Ong, W.-Y., Jenner, A.M., Farooqui, A.A., Patel, S.C. (2009). Apolipoprotein D modulates F2-isoprostane and 7-ketocholesterol formation and has a neuroprotective effect on organotypic hippocampal cultures after kainate-induced excitotoxic injury. Neuroscience Letters 455 (3) : 183-186. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neulet.2009.03.038
Abstract: Apolipoprotein D (apoD), a member of the lipocalin family of transporter proteins binds a number of small lipophilic molecules including arachidonic acid and cholesterol. Recent studies showed a protective function of mammalian apoD as well as its insect and plant homologs against oxidative stress. In this study we investigated the effect of direct addition of exogenous human apoD protein purified from breast cystic fluid to rat hippocampal slice cultures after excitotoxic injury induced by the glutamate analog kainate. ApoD at a concentration of 10 μg/ml partially prevented loss of MAP2 immunostaining and LDH release from injured hippocampal neurons after kainate injury. ApoD also attenuated the increase in oxidative products of arachidonic acid and cholesterol, F2-isoprostanes and 7-ketocholesterol, respectively, after kainate treatment. In view of the molecular structure of apoD which consists of an eight stranded β barrel that forms a binding pocket for a number of small hydrophobic molecules, we propose that apoD promotes its neuroprotective effects by binding to arachidonic acid and cholesterol thus preventing their oxidation to neurotoxic products such as 4-hydroxynonenal (4-HNE) and 7-ketocholesterol. © 2009 Elsevier Ireland Ltd. All rights reserved.
Source Title: Neuroscience Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/24366
ISSN: 03043940
DOI: 10.1016/j.neulet.2009.03.038
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