Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cbpc.2008.06.003
Title: Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi
Authors: Gao, R. 
Gopalakrishnakone, P. 
Keywords: CD44
Hyaluronidase
N-terminal sequence
Purification
Scorpion venom
Issue Date: 2008
Source: Gao, R.,Gopalakrishnakone, P. (2008). Isolation and characterization of a hyaluronidase from the venom of Chinese red scorpion Buthus martensi. Comparative Biochemistry and Physiology - C Toxicology and Pharmacology 148 (3) : 250-257. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cbpc.2008.06.003
Abstract: A hyaluronidase, named BmHYA1, was purified from the venom of Chinese red scorpion (Buthus martensi), using successive chromatography. The homogeneity of BmHYA1 was confirmed by SDS-PAGE and MALDI-TOF mass spectrometry. The molecular mass of BmHYA1 was 48,696 Da determined by MALDI-TOF MS. The optimal temperature and pH of BmHYA1 were 50 °C and pH 4.5, respectively. It could be inhibited by DTT, Cu2+, Fe3+ or heparin, but not Mg2+, Ca2+, reduced glutathione, l-cysteine or EDTA. The sequence of thirty N-terminal amino acids of BmHYA1 was obtained by Edman degradation, as TSADF KVVWE VPSIM CSKKF KICVT DLLTS; but no similarity was found to other venom hyaluronidases. Further, BmHYA1 can hydrolyze hyaluronan into relatively smaller oligosaccharides and modulate the expression of CD44 variant in the breast cancer cell line MDA-MB-231. © 2008 Elsevier Inc. All rights reserved.
Source Title: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
URI: http://scholarbank.nus.edu.sg/handle/10635/24314
ISSN: 15320456
DOI: 10.1016/j.cbpc.2008.06.003
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