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https://doi.org/10.1104/pp.20.01054
Title: | Regulation of a Cytochrome P450 Gene CYP94B1 by WRKY33 Transcription Factor Controls Apoplastic Barrier Formation in Roots to Confer Salt Tolerance | Authors: | Krishnamurthy, Pannaga Vishal, Bhushan Ho, Wan Jing Lok, Felicia Chien Joo Lee, Felicia Si Min Kumar, Prakash P |
Keywords: | Science & Technology Life Sciences & Biomedicine Plant Sciences RICE ROOTS ARABIDOPSIS SUBERIN EXPRESSION RESPONSES PLANTS WATER PERMEABILITIES TRANSFORMATION INACTIVATION |
Issue Date: | 1-Dec-2020 | Publisher: | OXFORD UNIV PRESS INC | Citation: | Krishnamurthy, Pannaga, Vishal, Bhushan, Ho, Wan Jing, Lok, Felicia Chien Joo, Lee, Felicia Si Min, Kumar, Prakash P (2020-12-01). Regulation of a Cytochrome P450 Gene CYP94B1 by WRKY33 Transcription Factor Controls Apoplastic Barrier Formation in Roots to Confer Salt Tolerance. PLANT PHYSIOLOGY 184 (4) : 2199-2215. ScholarBank@NUS Repository. https://doi.org/10.1104/pp.20.01054 | Abstract: | Salinity is an environmental stress that causes decline in crop yield. Avicennia officinalis and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from A. officinalis, AoCYP94B1, and its putative ortholog in Arabidopsis (Arabidopsis thaliana), AtCYP94B1, which are involved in apoplastic barrier formation. Both genes were induced by 30 min of salt treatment in the roots. Heterologous expression of AoCYP94B1 in the atcyp94b1 Arabidopsis mutant and wild-type rice (Oryza sativa) conferred increased NaCl tolerance to seedlings by enhancing root suberin deposition. Histochemical staining and gas chromatography-tandem mass spectrometry quantification of suberin precursors confirmed the role of CYP94B1 in suberin biosynthesis. Using chromatin immunoprecipitation and yeast one-hybrid and luciferase assays, we identified AtWRKY33 as the upstream regulator of AtCYP94B1 in Arabidopsis. In addition, atwrky33 mutants exhibited reduced suberin and salt-sensitive phenotypes, which were rescued by expressing 35S::AtCYP94B1 in the atwrky33 background. This further confirmed that AtWRKY33-mediated regulation of AtCYP94B1 is part of the salt tolerance mechanism. Our findings may help efforts aimed at generating salt-tolerant crops. | Source Title: | PLANT PHYSIOLOGY | URI: | https://scholarbank.nus.edu.sg/handle/10635/241953 | ISSN: | 0032-0889 1532-2548 |
DOI: | 10.1104/pp.20.01054 |
Appears in Collections: | Staff Publications Elements |
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Plant Physiol-2020-Pannaga CYP paper.pdf | Published version | 3.33 MB | Adobe PDF | CLOSED | Published |
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