Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.scr.2008.02.002
Title: Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium
Authors: Timmers, L.
Arslan, F.
Hoefer, I.E.
Doevendans, P.A.
Pasterkamp, G.
de, Kleijn D.P.V.
Lim, S.K. 
Armstrong, J.S. 
Piek, J.J.
El, Oakley R.M. 
Choo, A.
Lee, C.N. 
Issue Date: 2008
Source: Timmers, L., Arslan, F., Hoefer, I.E., Doevendans, P.A., Pasterkamp, G., de, Kleijn D.P.V., Lim, S.K., Armstrong, J.S., Piek, J.J., El, Oakley R.M., Choo, A., Lee, C.N. (2008). Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium. Stem Cell Research 1 (2) : 129-137. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2008.02.002
Abstract: Although paracrine effects of mesenchymal stem cells (MSCs) have been suggested previously, cardioprotection by human MSC secretions has never been demonstrated. Human MSC-conditioned medium (CM) was collected by following a clinically compliant protocol. In a porcine model of ischemia and reperfusion injury, intravenous and intracoronary MSC-CM treatment significantly reduced myocardial nuclear oxidative stress as determined by immunostaining for 8-hydroxy-2′-deoxyguanosine. In addition, expression levels of phospho-SMAD2 and active caspase 3 were diminished following CM treatment, suggesting that TGF-β signaling and apoptosis were reduced. This was associated with a 60% reduction in infarct size and marked improvement of systolic and diastolic cardiac performance as assessed with echocardiography and pressure volume loops. Fractionation studies revealed that only the fraction of the CM containing products > 1000 kDa (100-220 nm) provided cardioprotection in a mouse model of ischemia and reperfusion injury. This indicates that the responsible paracrine factor of human MSCs is likely a large complex rather than a single small molecule. These data identify human MSC-CM as a promising therapeutic option to reduce myocardial infarct size in patients with acute MI and suggest that the use of stem cell secretions could extend the applicability of stem cells for therapeutic purposes. © 2008 Elsevier B.V. All rights reserved.
Source Title: Stem Cell Research
URI: http://scholarbank.nus.edu.sg/handle/10635/24190
ISSN: 18735061
DOI: 10.1016/j.scr.2008.02.002
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

205
checked on Dec 13, 2017

WEB OF SCIENCETM
Citations

98
checked on Nov 16, 2017

Page view(s)

256
checked on Dec 10, 2017

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.