Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.canlet.2008.10.038
DC Field | Value | |
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dc.title | Silencing the Metallothionein-2A gene inhibits cell cycle progression from G1- to S-phase involving ATM and cdc25A signaling in breast cancer cells | |
dc.contributor.author | Lim, D. | |
dc.contributor.author | Jocelyn, K.M.-X. | |
dc.contributor.author | Yip, G.W.-C. | |
dc.contributor.author | Bay, B.-H. | |
dc.date.accessioned | 2011-07-18T08:44:28Z | |
dc.date.available | 2011-07-18T08:44:28Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Lim, D., Jocelyn, K.M.-X., Yip, G.W.-C., Bay, B.-H. (2009). Silencing the Metallothionein-2A gene inhibits cell cycle progression from G1- to S-phase involving ATM and cdc25A signaling in breast cancer cells. Cancer Letters 276 (1) : 109-117. ScholarBank@NUS Repository. https://doi.org/10.1016/j.canlet.2008.10.038 | |
dc.identifier.issn | 03043835 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/23911 | |
dc.description.abstract | Metallothioneins (MTs) are a group of metal-binding proteins involved in cell proliferation, differentiation and apoptosis. The MT-2A isoform is generally the most abundant isoform among the 10 known functional MT genes. In the present study, we observed that down-regulation of the MT-2A gene in MCF-7 cells via siRNA-mediated silencing inhibited cell growth by inducing cell cycle arrest in G1-phase (G1-arrest) and a marginal increase in cells in sub-G1-phase. Scanning electron microscopic examination of the cells with silenced expression of MT-2A (siMT-2A cells) revealed essentially normal cell morphology with presence of scattered apoptotic cells. To elucidate the underlying molecular mechanism, we examined the expression of cell cycle related genes in MT-2A-silenced cells and found a higher expression of the ataxia telangiectasia mutated (ATM) gene concomitant with a lower expression of the cdc25A gene. These data suggest that MT-2A could plausibly modulate cell cycle progression from G1- to S-phase via the ATM/Chk2/cdc25A pathway. © 2008 Elsevier Ireland Ltd. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.canlet.2008.10.038 | |
dc.source | Scopus | |
dc.subject | ATM | |
dc.subject | Breast cancer | |
dc.subject | cdc25A | |
dc.subject | Cell cycle | |
dc.subject | Metallothionein | |
dc.type | Article | |
dc.contributor.department | ANATOMY | |
dc.description.doi | 10.1016/j.canlet.2008.10.038 | |
dc.description.sourcetitle | Cancer Letters | |
dc.description.volume | 276 | |
dc.description.issue | 1 | |
dc.description.page | 109-117 | |
dc.identifier.isiut | 000264581900016 | |
Appears in Collections: | Staff Publications |
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