Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.canlet.2008.10.038
Title: Silencing the Metallothionein-2A gene inhibits cell cycle progression from G1- to S-phase involving ATM and cdc25A signaling in breast cancer cells
Authors: Lim, D.
Jocelyn, K.M.-X.
Yip, G.W.-C. 
Bay, B.-H. 
Keywords: ATM
Breast cancer
cdc25A
Cell cycle
Metallothionein
Issue Date: 2009
Source: Lim, D.,Jocelyn, K.M.-X.,Yip, G.W.-C.,Bay, B.-H. (2009). Silencing the Metallothionein-2A gene inhibits cell cycle progression from G1- to S-phase involving ATM and cdc25A signaling in breast cancer cells. Cancer Letters 276 (1) : 109-117. ScholarBank@NUS Repository. https://doi.org/10.1016/j.canlet.2008.10.038
Abstract: Metallothioneins (MTs) are a group of metal-binding proteins involved in cell proliferation, differentiation and apoptosis. The MT-2A isoform is generally the most abundant isoform among the 10 known functional MT genes. In the present study, we observed that down-regulation of the MT-2A gene in MCF-7 cells via siRNA-mediated silencing inhibited cell growth by inducing cell cycle arrest in G1-phase (G1-arrest) and a marginal increase in cells in sub-G1-phase. Scanning electron microscopic examination of the cells with silenced expression of MT-2A (siMT-2A cells) revealed essentially normal cell morphology with presence of scattered apoptotic cells. To elucidate the underlying molecular mechanism, we examined the expression of cell cycle related genes in MT-2A-silenced cells and found a higher expression of the ataxia telangiectasia mutated (ATM) gene concomitant with a lower expression of the cdc25A gene. These data suggest that MT-2A could plausibly modulate cell cycle progression from G1- to S-phase via the ATM/Chk2/cdc25A pathway. © 2008 Elsevier Ireland Ltd. All rights reserved.
Source Title: Cancer Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/23911
ISSN: 03043835
DOI: 10.1016/j.canlet.2008.10.038
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