Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23242
Title: The role of CDC42, 1RSP53 and its binding partners in filopodia formation
Authors: LIM KIM BUAY
Keywords: Cdc42 IRSp53 Filopodia IMD N-WASP SH3
Issue Date: 30-Aug-2007
Source: LIM KIM BUAY (2007-08-30). The role of CDC42, 1RSP53 and its binding partners in filopodia formation. ScholarBank@NUS Repository.
Abstract: The Cdc42 effector IRSp53 is a strong inducer of filopodia formation. It consists of an SH3 domain, a potential WW binding motif, a partial-CRIB motif, and an IMD domain (IRSp53 and Missing in metastasis homology Domain). In this study, I show that IRSp53 interacts directly with N-WASP via its SH3 domain. Furthermore, N-WASP is required for filopodia formation as IRSp53 failed to induce filopodia formation in N-WASP knock out (KO) fibroblasts. IRSp53-induced filopodia formation is reconstituted in N-WASP KO fibroblasts by full length N-WASP and by N-WASP-WA (mutant unable to activate Arp2/3 complex). IRSp53 also failed to induce filopodia in Mena/VASP KO cells. The IRSp53 IMD domain alone induces a??partial-filopodiaa?? (dynamic membrane protrusions dissociated from actin dynamics), in both wild-type N-WASP and N-WASP KO cells. I propose that IRSp53 generates filopodia by coupling membrane protrusion through its IMD domain with actin dynamics through SH3 domain binding partners N-WASP and Mena.
URI: http://scholarbank.nus.edu.sg/handle/10635/23242
Appears in Collections:Ph.D Theses (Open)

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