Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23221
Title: siRNA, miRNA and their possible roles in molecular therapy of chronic human hepatitis B virus infection and hepatocellular carcinoma
Authors: LI YANG
Keywords: Short interfering RNA, microRNA, hepatocellular carcinoma, hepatitis B, expression profiling, cDNA microarray
Issue Date: 1-Sep-2007
Source: LI YANG (2007-09-01). siRNA, miRNA and their possible roles in molecular therapy of chronic human hepatitis B virus infection and hepatocellular carcinoma. ScholarBank@NUS Repository.
Abstract: The study aims to investigate the therapeutic and diagnostic potential of small RNA molecules in hepatic diseases. Small interfering RNAs (siRNAs) targeting HBsAg inhibited HBsAg producing on PLC/PRF/5 and 2.2.15 cells by up to 96.3% and virion production in 2.2.15 cells by up to 71.8%. MTS assay showed that cell growth and viability were not affected and cDNA microarray assay observed no significant off-target gene regulation. Our findings indicate that siRNA can be specific in mediating down-regulation of viral gene expression and virion production. miRNA expression profiling using reverse transcription-real-time PCR was carried out in paired HCC tumor and non-tumor samples and showed that of the 157 miRNAs examined, 28 were upregulated while 14 were downregulated. The knock-down studies in cell lines for the upregulated miR-106b-125 cluster showed that the cluster is necessary for cell proliferation and for the anchorage-independent growth, indicating that the miR-106b-125 cluster may have oncogenic properties.
URI: http://scholarbank.nus.edu.sg/handle/10635/23221
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