Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23200
Title: Investigation of suppressor of overexpression of constans 1 (SOC1) function in flowering time control of Arabidopsis thaliana
Authors: CHEN HONGYAN
Keywords: Arabidopsis, flowering, SOC1, SVP, AGL24, LFY
Issue Date: 28-Nov-2007
Source: CHEN HONGYAN (2007-11-28). Investigation of suppressor of overexpression of constans 1 (SOC1) function in flowering time control of Arabidopsis thaliana. ScholarBank@NUS Repository.
Abstract: The objective of this study is to clarify the SUPPRESSOR OF OVEREXPRESSION OF CO 1 (SOC1)-mediated regulatory network in the flowering time control of Arabidopsis.Two homologous genes, SVP (SHORT VEGETATIVE PHASE) and AGL24 (AGAMOUS LIKE 24) have been identified as SOC1 regulators. Real-time RT-PCR results indicated that SVP constantly and dominantly represses SOC1 since a very early stage of the vegetative phase, while AGL24 promotes SOC1 expression in a temporally restricted manner. Furthermore, ChIP assays suggest these regulations are direct and imply the existence of feedback regulation.Meanwhile, a 35S:SOC1-9myc tagging system was also used to investigate downstream target genes of SOC1. It was found that SOC1-9myc fusion protein binds to the genomic sequence of LEAFY (LFY), a key floral meristem identity gene in Arabidopsis thaliana. This is the first set of biochemical evidence for direct flowering signal transduction from SOC1 to LFY.
URI: http://scholarbank.nus.edu.sg/handle/10635/23200
Appears in Collections:Master's Theses (Open)

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