Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23179
Title: Distinct histone deacetylases repress expression of LH and FSH beta genes in the immature gonadotrope alphaT3-1 cells and the repression is reversed by GNRH
Authors: YANG MENG
Keywords: HISTONE DEACETYLASES REPRESS LH-BETA FSH-BETA GNRH
Issue Date: 8-Jul-2007
Source: YANG MENG (2007-07-08). Distinct histone deacetylases repress expression of LH and FSH beta genes in the immature gonadotrope alphaT3-1 cells and the repression is reversed by GNRH. ScholarBank@NUS Repository.
Abstract: The gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are synthesized in and secreted by the pituitary gland, and play crucial roles in regulating reproduction. The synthesis of both LH and FSH is repressed soon after birth until puberty, when the repression is reversed by gonadotropin-releasing hormone (GnRH). Chromatin immunoprecipitation (ChIP) assays have shown that in the immature pituitary gonadotrope I?T3-1 cells, distinct sets of histone deacetylases (HDACs), along with SMRT and Sin3A, associate with LH and/or FSH I?-subunit gene promoters in a repressive complex. In order to understand the de-repression of LH and FSH I? genes in I?T3-1 cells, the effect of GnRH treatment on this repressive complex must be elucidated. ChIP assays have shown that GnRH is able to remove several HDACs and Sin3A from LH and/or FSH I? gene promoters, which results in the disruption of the repressive complex. De-repression of the LH and FSH I? genes after GnRH stimulation might be caused by class IIa HDAC modifications which lead to the nuclear export of those HDACs. SENP1, a nuclear protease that appears to deconjugate sumoylated proteins, reverses the repression of both the LH and FSH I? genes in the I?T3-1 cells. It is possible that GnRH stimulation recruits the de-sumoylation pathway to export the class IIa HDACs from the nucleus resulting in the disruption of the repressive complex.
URI: http://scholarbank.nus.edu.sg/handle/10635/23179
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