Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23176
Title: Post-translational regulation of the human TRIP-Br1 cell cycle protein
Authors: YANG MAOLIN, CHRISTOPHER
Keywords: cell cycle, TRIP-Br, transcriptional regulator, PHD, bromodomain, DP1
Issue Date: 26-Oct-2007
Source: YANG MAOLIN, CHRISTOPHER (2007-10-26). Post-translational regulation of the human TRIP-Br1 cell cycle protein. ScholarBank@NUS Repository.
Abstract: The TRIP-Br proteins are a novel family of transcriptional regulators proposed to function as a??integratorsa?? at E2F responsive promoters to integrate signals provided by PHD zinc finger- and/or bromodomain-containing transcription factors. Co-immunoprecipitation assays with exogenously co-overexpressed proteins were used to determine interactions between hTRIP-Br1-HA and DP1, E2F1 and/or p53. A putative region for binding to DP1 on hTRIP-Br1 was identified. Conversely, the DP1 DCB1 domain was postulated to be the binding region for hTRIP-Br1,in a review of published data. p53 was also observed to be a component of the E2F1/DP1/hTRIP-Br1-HA quaternary complex.TRIP-Br1 binding partners affected its intracellular protein levels. DP1 interaction with hTRIP-Br1-HA resulted in elevated intracellular protein levels. E2F1 accelerated the turnover of hTRIP-Br1-HA, with or without DP1The results support a regulatory model in which hTRIP-Br1-HA is able to promote DP1 nuclear localization through post-translational mechanisms in a reciprocal manner that influences the activities of both proteins.
URI: http://scholarbank.nus.edu.sg/handle/10635/23176
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Final Thesis - Christopher Yang, PhD, Dept of Medicine, 2007.pdf9.06 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

187
checked on Dec 11, 2017

Download(s)

163
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.