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Title: | Post-translational regulation of the human TRIP-Br1 cell cycle protein | Authors: | YANG MAOLIN, CHRISTOPHER | Keywords: | cell cycle, TRIP-Br, transcriptional regulator, PHD, bromodomain, DP1 | Issue Date: | 26-Oct-2007 | Citation: | YANG MAOLIN, CHRISTOPHER (2007-10-26). Post-translational regulation of the human TRIP-Br1 cell cycle protein. ScholarBank@NUS Repository. | Abstract: | The TRIP-Br proteins are a novel family of transcriptional regulators proposed to function as a??integratorsa?? at E2F responsive promoters to integrate signals provided by PHD zinc finger- and/or bromodomain-containing transcription factors. Co-immunoprecipitation assays with exogenously co-overexpressed proteins were used to determine interactions between hTRIP-Br1-HA and DP1, E2F1 and/or p53. A putative region for binding to DP1 on hTRIP-Br1 was identified. Conversely, the DP1 DCB1 domain was postulated to be the binding region for hTRIP-Br1,in a review of published data. p53 was also observed to be a component of the E2F1/DP1/hTRIP-Br1-HA quaternary complex.TRIP-Br1 binding partners affected its intracellular protein levels. DP1 interaction with hTRIP-Br1-HA resulted in elevated intracellular protein levels. E2F1 accelerated the turnover of hTRIP-Br1-HA, with or without DP1The results support a regulatory model in which hTRIP-Br1-HA is able to promote DP1 nuclear localization through post-translational mechanisms in a reciprocal manner that influences the activities of both proteins. | URI: | http://scholarbank.nus.edu.sg/handle/10635/23176 |
Appears in Collections: | Ph.D Theses (Open) |
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Final Thesis - Christopher Yang, PhD, Dept of Medicine, 2007.pdf | 9.06 MB | Adobe PDF | OPEN | None | View/Download |
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