Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23138
Title: Investigating the role of menaquinone metabolism in dormant mycobacteria with antisense RNA
Authors: THOMAS MICHAEL FIEDLER
Keywords: mycobacteria, menaquinone, dormancy, antisense, tuberculosis, respiration
Issue Date: 29-May-2007
Source: THOMAS MICHAEL FIEDLER (2007-05-29). Investigating the role of menaquinone metabolism in dormant mycobacteria with antisense RNA. ScholarBank@NUS Repository.
Abstract: Mycobacteria exclusively use menaquinones in their respiratory chain. The fact that humans rely on ubiquinone and do not have the capability to synthesize menaquinones renders menaquinone metabolism an attractive drug target. We investigate here if menaquinone is essential for bacterial survival during dormancy, by inhibiting the translation of genes coding for menaquinone synthesizing enzymes, through the experimental use of antisense RNA. Differences between transformants and wild-type, concerning the bacteriaa??s ability to survive hypoxia and synthesize menaquinone, were monitored by counting colony forming units (CFU) and measuring levels of menaquinones via mass spectrometry (MS). Based on our observations of cell growth, cells transformed with a plasmid carrying an antisense fragment of the gene entC were compromised in their ability to survive hypoxic conditions. However, an inhibition of menaquinone synthesis and concurrent drops in menaquinone levels could not be confirmed by preliminary MS analysis.
URI: http://scholarbank.nus.edu.sg/handle/10635/23138
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Amendments Thomas Fiedler 130507.doc26.5 kBMicrosoft Word

OPEN

NoneView/Download
Thomas Fiedler amended thesis 160507.pdf1.09 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

199
checked on Dec 11, 2017

Download(s)

48
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.