Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23097
Title: Colloidal crystals of nanospheres for proteIn entrapment
Authors: YEON WEN CONG
Keywords: Self-assembly, colloidal crystals, horizontal deposition, fluorescence confocal microscopy, single molecule detection
Issue Date: 22-Nov-2007
Source: YEON WEN CONG (2007-11-22). Colloidal crystals of nanospheres for proteIn entrapment. ScholarBank@NUS Repository.
Abstract: Colloidal crystals from nanospheres stand out as an excellent alternative to conventional photolithography techniques to fabricate three-dimensional regular repeating structures. Self-assembly is capable of forming nanometer features in a low cost, high throughput fashion. In this thesis, a novel biological application of self-assembled colloidal crystals is successfully demonstrated: Confinement of protein in the interstitial cavities of the colloidal crystals. Colloidal crystals were fabricated from a modified horizontal deposition method which increases the packing efficiency of the self-assembly mechanism. Confinement provided by the cavities was assessed through computer visualization and mathematical calculations. Fluorescence confocal spectroscopy was used to study the diffusion and confinement of molecules inside the colloidal crystals. Using scanning confocal microscopy, entrapment of single enzyme molecules was shown and we observe the enzyme turning substrates into products. Possible applications include scientific studies of the effects of protein confinement on protein stability, pharmaceutical protein microarrays and three-dimensional optical bio-memories.
URI: http://scholarbank.nus.edu.sg/handle/10635/23097
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