Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23092
Title: Cell-cell communication in xanthomonas campestris pv. campestris
Authors: HE YAWEN
Keywords: Xanthomonas campestris, DSF, Cell-cell communication, regulatory network.
Issue Date: 13-Feb-2007
Source: HE YAWEN (2007-02-13). Cell-cell communication in xanthomonas campestris pv. campestris. ScholarBank@NUS Repository.
Abstract: Xanthomonas campestris pv. campestris (Xcc) is a major bacterial pathogen of cruciferous plants worldwide. The production of Xcc virulence factors is regulated by a diffusible signal factor DSF. In this study, DSF was purified and chemically characterized as cis-11-methyl-2-dodecenoic acid. Oligomicroarray analysis of Xcc wild-type and DSF deficient mutant led to identification of 165 genes, whose expression is significantly influenced by DSF signals. These genes encode proteins and enzymes belonging to at least 12 functional groups. In addition to those previously known DSF-dependent activities such as production of extracellular enzymes and extracellular polysaccharides, microarray analyses also revealed new functions mediated by DSF, such as flagellum synthesis, resistance to toxins and oxidative stress, and aerobic respiration. The hybrid sensor RpfC positively regulates the expression of a range of virulence genes and negatively modulates the synthesis of quorum sensing signal DSF by utilization of a conserved phosphorelay system and a novel domain-specific protein-protein interaction mechanism, respectively. RpfG, which functions as cyclic di-GMP turnover, is the cognate response regulator of RpfC. Further studies showed that DSF signaling involves a nucleotide receptor protein Clp and a hierarchical signaling network.
URI: http://scholarbank.nus.edu.sg/handle/10635/23092
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