Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23073
Title: A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins
Authors: LUO MIN
Keywords: GnRH, gonadotropin, ubc4, calcineurin
Issue Date: 28-Feb-2007
Source: LUO MIN (2007-02-28). A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins. ScholarBank@NUS Repository.
Abstract: The pituitary gonadotropes synthesize and secrete luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which control reproductive development and function. In mature gonadotropes and the LI?T2 cell line, both hormones are regulated by GnRH, but the hormone-specific I? subunits are not expressed in the I?T3-1 cells, which represents an immature gonadotrope. In order to identify novel factors and mechanisms involved in basic and GnRH-induced gonadotropin genes transcription, subtractive hybridization was carried out to reveal genes expressed in mature LI?T2 but not in immature I?T3-1 cells, or those whose expression in LI?T2 cells are induced following GnRH treatment. A number of candidate genes was identified, among them the ubiquitin-conjugating enzyme ubc4, and calmodulin-dependent serine/threonine protein phosphatase calcineurin, both of which are up-regulated following GnRH treatment. Further functional studies revealed that ubc4 takes part in the degradation of estrogen receptor I? (ERI?), and enhances ERI? transactivation of LHI?; while calcineurin mediates GnRH stimulatory effects on the I?GSU subunit, and de-repression of FSHI? subunit gene in immature gonadotrope.
URI: http://scholarbank.nus.edu.sg/handle/10635/23073
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