Please use this identifier to cite or link to this item:
Title: A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins
Authors: LUO MIN
Keywords: GnRH, gonadotropin, ubc4, calcineurin
Issue Date: 28-Feb-2007
Citation: LUO MIN (2007-02-28). A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins. ScholarBank@NUS Repository.
Abstract: The pituitary gonadotropes synthesize and secrete luteinizing hormone (LH) and follicle-stimulating hormone (FSH), which control reproductive development and function. In mature gonadotropes and the LI?T2 cell line, both hormones are regulated by GnRH, but the hormone-specific I? subunits are not expressed in the I?T3-1 cells, which represents an immature gonadotrope. In order to identify novel factors and mechanisms involved in basic and GnRH-induced gonadotropin genes transcription, subtractive hybridization was carried out to reveal genes expressed in mature LI?T2 but not in immature I?T3-1 cells, or those whose expression in LI?T2 cells are induced following GnRH treatment. A number of candidate genes was identified, among them the ubiquitin-conjugating enzyme ubc4, and calmodulin-dependent serine/threonine protein phosphatase calcineurin, both of which are up-regulated following GnRH treatment. Further functional studies revealed that ubc4 takes part in the degradation of estrogen receptor I? (ERI?), and enhances ERI? transactivation of LHI?; while calcineurin mediates GnRH stimulatory effects on the I?GSU subunit, and de-repression of FSHI? subunit gene in immature gonadotrope.
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Thesis compiled.pdf4.92 MBAdobe PDF



Page view(s)

checked on Nov 24, 2018


checked on Nov 24, 2018

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.