Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/23051
Title: The study of gene and protein vaccines for allergic diseases in mice
Authors: TAN LI KIANG
Keywords: allergen, Der p 2, DNA vaccine, immunotherapy, dosage
Issue Date: 10-Aug-2007
Source: TAN LI KIANG (2007-08-10). The study of gene and protein vaccines for allergic diseases in mice. ScholarBank@NUS Repository.
Abstract: Allergen-specific immunotherapy is curative treatment for allergic diseases. To understand the mechanisms of prophylactic and therapeutic treatments for allergic diseases, this study examines the immunological responses and the mechanisms induced in protein and gene immunization in mice. Results show that mice primed with 10 A?g (D10) of the Der p 2 allergen induce a strong Th2 response, while those primed with 50 A?g (D50) of the Der p 2 allergen induce the development of Treg cells, which might hinder the IL-13 dependent IgE synthesis. Microarray profiling identifies several genes that may be associated with the immunological responses of the protein immunized mice. Der p 2 gene vaccinations in mice demonstrate the potential risk of incorporating a strong signal sequence that could provoke a masked Th2 response. The inclusion of lysosomal-targeting sequences in the gene construct may improve the safety of allergen vaccines. Overall, these results provide critical information for future development of new vaccination strategies.
URI: http://scholarbank.nus.edu.sg/handle/10635/23051
Appears in Collections:Ph.D Theses (Open)

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