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https://scholarbank.nus.edu.sg/handle/10635/22622
DC Field | Value | |
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dc.title | Study of genome packaging using single-molecule manipulation and image method | |
dc.contributor.author | LIU YINGJIE | |
dc.date.accessioned | 2011-05-12T18:00:07Z | |
dc.date.available | 2011-05-12T18:00:07Z | |
dc.date.issued | 2009-10-14 | |
dc.identifier.citation | LIU YINGJIE (2009-10-14). Study of genome packaging using single-molecule manipulation and image method. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/22622 | |
dc.description.abstract | In this thesis, I will describe 3 kinds of proteins that have been studied in my lab:Integration Host Factor (IHF), VP15 from White Spot Syndrome Virus (WSSV) and Histone-like Nucleoid Structural Protein (H-NS). All of them are DNA binding protein and have large influence on the DNA topography. Our main interest is placed on the topography of DNA-protein complexes, critical folding force and protein function under different ionic condition. VP15 shows the strongest DNA compacting ability among the 3 kinds of proteins with a critical folding force up to 5 pN. However, IHF and H-NS are more interesting than VP15 in that their functions are ionic concentration dependent. The bending ability of scIHF, presented by bending angle distribution of DNA-IHF structure, depends on Mg2+ concentration. The most notable protein, H-NS, shows two switchable functioning modes according to whether Mg2+ or Ca2+ concentration is above certain value and two distinctly different DNA-H-NS structures are found using AFM. | |
dc.language.iso | en | |
dc.subject | DNA, AFM, Magnetic Tweezer | |
dc.type | Thesis | |
dc.contributor.department | PHYSICS | |
dc.contributor.supervisor | YAN JIE | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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STUDY OF GENOME PACKAGING USING SINGLE-MOLECULE MANIPULATION AND IMAGE METHOD.pdf | 5.13 MB | Adobe PDF | OPEN | None | View/Download |
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