Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/22622
Title: Study of genome packaging using single-molecule manipulation and image method
Authors: LIU YINGJIE
Keywords: DNA, AFM, Magnetic Tweezer
Issue Date: 14-Oct-2009
Source: LIU YINGJIE (2009-10-14). Study of genome packaging using single-molecule manipulation and image method. ScholarBank@NUS Repository.
Abstract: In this thesis, I will describe 3 kinds of proteins that have been studied in my lab:Integration Host Factor (IHF), VP15 from White Spot Syndrome Virus (WSSV) and Histone-like Nucleoid Structural Protein (H-NS). All of them are DNA binding protein and have large influence on the DNA topography. Our main interest is placed on the topography of DNA-protein complexes, critical folding force and protein function under different ionic condition. VP15 shows the strongest DNA compacting ability among the 3 kinds of proteins with a critical folding force up to 5 pN. However, IHF and H-NS are more interesting than VP15 in that their functions are ionic concentration dependent. The bending ability of scIHF, presented by bending angle distribution of DNA-IHF structure, depends on Mg2+ concentration. The most notable protein, H-NS, shows two switchable functioning modes according to whether Mg2+ or Ca2+ concentration is above certain value and two distinctly different DNA-H-NS structures are found using AFM.
URI: http://scholarbank.nus.edu.sg/handle/10635/22622
Appears in Collections:Ph.D Theses (Open)

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