Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/20903
Title: Tissue Engineering Approach for Annulus Fibrosus Regeneration
Authors: SEE YONG-SHUN, EUGENE
Keywords: Tissue Engineering, Intervertebral Disc, Annulus Fibrosus, Bone Marrow Stem Cells, Cell-Sheet
Issue Date: 24-Sep-2010
Source: SEE YONG-SHUN, EUGENE (2010-09-24). Tissue Engineering Approach for Annulus Fibrosus Regeneration. ScholarBank@NUS Repository.
Abstract: The aim of this study was to develop a tissue engineering approach in regenerating the annulus fibrosus (AF) as part of an overall strategy to produce a tissue-engineered intervertebral disc replacement. The approach was to use bone marrow derived stem cells (BMSC) to form cell-sheets and incorporating them onto silk scaffolds to simulate the native lamellae of the AF. The in vitro experimental model used to study the efficacy of such a system was made up of the tissue engineering AF construct wrapped around a silicone disc to form a simulated IVD-like assembly. The AF construct was cultured within a custom-designed bioreactor that provided a mechanical stimulation to mimic the physiological condition. The results showed that BMSC cell-sheets retain their multipotentiality and were a suitable cell source for the simulated AF. The use of the bioreactor on the experimental model was shown to further enhance the efficacy in regenerating the inner AF.
URI: http://scholarbank.nus.edu.sg/handle/10635/20903
Appears in Collections:Ph.D Theses (Open)

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