Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/20803
Title: In vitro-bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation
Authors: PENG YANXIAN
Keywords: human embryonic stem cells, feeder-free culture, extracelluar matrix, macromolecular crowding, chemically defined medium
Issue Date: 11-Aug-2010
Source: PENG YANXIAN (2010-08-11). In vitro-bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation. ScholarBank@NUS Repository.
Abstract: Human embryonic stem (hES) cells can be applied in tissue engineering, transplant therapy and drug discovery. However, traditional cultures of hES cells on feeders or mouse-origin Matrigel are not transplant-friendly as they pose a xenogenic threat or add technical challenges. Bioassembled human cell-derived matrices would be more ideal and serve as an in vivo model of ECM for hES cells. Macromolecular crowders applied to fibroblast cultures resulted in deposition of collagenous ECM, which were decellularized by detergent treatments. By varying the macromolecule used and the detergents, 5 matrix types were produced, which retained collagen I, fibronectin and various other proteins. Compared to control hES cells cultured on Matrigel, matrix-cultured hES cells achieved superior population doublings. These hES cells maintained pluripotent marker expressions, while retaining differentiation capacity, and retained normal karyotypes despite extended propagation. Hence, a cell-free bioassembled matrix has been developed that is suitable for hES cell culture.
URI: http://scholarbank.nus.edu.sg/handle/10635/20803
Appears in Collections:Ph.D Theses (Open)

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