Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/19240
Title: Interaction of Human umbilical vein endothelial cells and human hepatocytes celline on Gelatin-VEGF Microspheres and PHBV-HGF Microspheres in co-culture
Authors: SULTANA RAHMAN
Keywords: Microspheres, Co-culture, Membrane, Growth Factors, Encapsulated
Issue Date: 18-Mar-2009
Source: SULTANA RAHMAN (2009-03-18). Interaction of Human umbilical vein endothelial cells and human hepatocytes celline on Gelatin-VEGF Microspheres and PHBV-HGF Microspheres in co-culture. ScholarBank@NUS Repository.
Abstract: This research focused on the fabrication of HGF encapsulated PHBV microspheres and VEGF encapsulated gelatin microspheres to ensure controlled release of growth factors as the microspheres degrade during cell culture. Blank microspheres and 2D cell cultures were used as controls. Characterization of the microspheres was done by SEM, in-vitro degradation study and in-vitro release of the encapsulated growth factors. Direct random co-culture and co-culture through semi-permeable membranes of HUVEC cells on Gelatin-VEGF microspheres and Hep3B cells on PHBV-HGF microspheres was done. Cells grown by direct random co-culture and co-culture through the membranes exhibited higher viability and high P-450 cythocrome activity throughout the experiment as opposed to the controls with the co-culture through membranes showing the highest activity. To conclude, co-culture of Hep3B and HUVEC cells on growth factor encapsulated microspheres through semi-permeable membranes showed the most desirable results as it most closely mimics the in vivo liver environment.
URI: http://scholarbank.nus.edu.sg/handle/10635/19240
Appears in Collections:Master's Theses (Open)

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Acknowledgements.pdf7.26 kBAdobe PDF

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TABLE OF CONTENTS.pdf15.43 kBAdobe PDF

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SUMMARY.pdf15.04 kBAdobe PDF

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LIST OF TABLES.pdf6.79 kBAdobe PDF

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LIST OF FIGURES.pdf13.26 kBAdobe PDF

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LIST OF SYMBOLS.pdf9.27 kBAdobe PDF

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CHAPTER 1 - INTRODUCTION.pdf27.8 kBAdobe PDF

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CHAPTER 2 - LITERATURE REVIEW.pdf476.3 kBAdobe PDF

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CHAPTER 3 - MATERIALS AND METHODS.pdf153.96 kBAdobe PDF

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CHAPTER 4 - RESULTS AND DISCUSSION.pdf3.18 MBAdobe PDF

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Chapter 5 - CONCLUSION AND RECOMMENDATIONS.pdf31.23 kBAdobe PDF

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REFERENCES.pdf87.11 kBAdobe PDF

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