Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/18901
Title: Development of a bioreactor for in-vitro engineering of soft tissues
Authors: KYAW MOE
Keywords: Cyclic uniaxial strain, bioreactor, fibroblasts, cell alignment, PLGA scaffolds, Ligaments
Issue Date: 20-Jan-2006
Source: KYAW MOE (2006-01-20). Development of a bioreactor for in-vitro engineering of soft tissues. ScholarBank@NUS Repository.
Abstract: Mechanical strain regulates the structure and mechanical properties of the engineering tissue. To investigate the effect of cyclic mechanical strain and strain rate, a bioreactor was designed and fabricated. This bioreactor was designed and developed to strain the cell seeded PLGA scaffolds in various strain values and strain frequencies. The system can strain with minimum frequency of 0.01Hz and minimum extension of 0.25mm on three knitted PLGA scaffolds of length varying from 50mm to 70 mm. In this research the cell morphology was studied by using two sets of straining frequencies( 1Hz and 0.1 Hz) with 1.8% strain for 4 hours daily. After two weeks stimulation, the mean nuclei lengths from both frequency sets are longer than unstrained specimens. The cell population also tended to orientate parallel to the straining axis. The presence of extra cellular matrix ( Collagen type I, type III and Tenascin-C) was also studied through the use of the immunohistochemistry in 0.1 Hz frequency set .
URI: http://scholarbank.nus.edu.sg/handle/10635/18901
Appears in Collections:Master's Theses (Open)

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