Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/18425
Title: Study of the Effect of Transforming Growth Factor ?-1 on the gap junction protein connexin 43 in hepatic stellate cells
Authors: LIM CHIN CHIA MICHELLE
Keywords: Transforming growth factor-ß1, Connexin 43, Snai1, Hepatic Stellate Cells
Issue Date: 4-Jun-2010
Source: LIM CHIN CHIA MICHELLE (2010-06-04). Study of the Effect of Transforming Growth Factor ?-1 on the gap junction protein connexin 43 in hepatic stellate cells. ScholarBank@NUS Repository.
Abstract: Hepatic stellate cells (HSCs) communicate to each other through functional gap junctions composed of connexin 43 (Cx43) proteins. Exogenous recombinant human TGF-?1 (rhTGF-?1), a pro-fibrotic stimulus, decreases Cx43 mRNA and protein as well as increases the phosphorylation of Cx43 at serine 368 in rat HSCs. These effects lead to a decrease in the gap junction intercellular communication between the HSCs, as shown by gap-FRAP analysis. Results showing that the transcription factor Snai1 can bind to its consensus sequence in the Cx43 promoter and Snai1 siRNA transfection causes an up-regulation of Cx43 imply that TGF-?1 may regulate Cx43 via Snai1. Furthermore, the knockdown of Cx43 by siRNA transfection leads to slower proliferation of HSCs. These findings suggest a new effect of TGF-?1 in HSCs, whereby it regulates intercellular communication by affecting the expression level and the phosphorylation state of Cx43 through Snai1 signaling.
URI: http://scholarbank.nus.edu.sg/handle/10635/18425
Appears in Collections:Master's Theses (Open)

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