Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/18408
Title: Development of micro-bioreactors for a more efficient fermentation process to produce bio-ethanol
Authors: TAN SOOK MUN
Keywords: yeast, microencapsulation, gellan gum, alginate, fermentation, bio-ethanol
Issue Date: 8-Jan-2010
Source: TAN SOOK MUN (2010-01-08). Development of micro-bioreactors for a more efficient fermentation process to produce bio-ethanol. ScholarBank@NUS Repository.
Abstract: Microencapsulation was employed to improve the fermentation efficiency of yeast. Using the emulsification method, yeast cells were successfully encapsulated using alginate or gellan gum to produce micro-bioreactors. The encapsulating polymer protected the yeast cells from the adverse environmental conditions produced during fermentation. Alginate produced weaker micro-bioreactors, whose stability could be enhanced by scaffold-coating. The ethanol yield of the yeast-gellan gum micro-bioreactors was comparable to that of the free yeast but the fermentation time required was longer. The micro-bioreactors served as a reservoir for the generation of yeast cells into the fermentation medium. Both the liberated and encapsulated yeast cells were involved in ethanol production. The longer fermentation time was attributed to the time taken for cell breakthrough and the impairment of mass transfer due to the encapsulating polymer matrix. Most importantly, the micro-bioreactors remained intact and could be re-used for at least fifteen fermentation cycles with relatively high ethanol yields.
URI: http://scholarbank.nus.edu.sg/handle/10635/18408
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