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https://doi.org/10.1186/1471-213X-10-46
Title: | Knockout of the folate transporter folt-1 causes germline and somatic defects in C. elegans | Authors: | Austin, M.U Liau, W.-S Balamurugan, K Ashokkumar, B Said, H.M Lamunyon, C.W |
Keywords: | Animalia Caenorhabditis elegans Mus Nematoda Caenorhabditis elegans protein folic acid Folt 1 protein, C elegans Folt-1 protein, C elegans organic anion transporter animal article Caenorhabditis elegans gene inactivation genetics germ cell hermaphroditism longevity male metabolism Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Folic Acid Gene Knockout Techniques Germ Cells Hermaphroditism Longevity Male Organic Anion Transporters |
Issue Date: | 2010 | Citation: | Austin, M.U, Liau, W.-S, Balamurugan, K, Ashokkumar, B, Said, H.M, Lamunyon, C.W (2010). Knockout of the folate transporter folt-1 causes germline and somatic defects in C. elegans. BMC Developmental Biology 10 : 46. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-213X-10-46 | Rights: | Attribution 4.0 International | Abstract: | Background. The C. elegans gene folt-1 is an ortholog of the human reduced folate carrier gene. The FOLT-1 protein has been shown to transport folate and to be involved in uptake of exogenous folate by worms. A knockout mutation of the gene, folt-1(ok1460), was shown to cause sterility, and here we investigate the source of the sterility and the effect of the folt-1 knockout on somatic function. Results. Our results show that folt-1(ok1460) knockout hermaphrodites have a substantially reduced germline, generate a small number of functional sperm, and only rarely produce a functional oocyte. We found no evidence of increased apoptosis in the germline of folt-1 knockout mutants, suggesting that germline proliferation is defective. While folt-1 knockout males are fertile, their rate of spermatogenesis was severely diminished, and the males were very poor maters. The mating defect is likely due to compromised metabolism and/or other somatic functions, as folt-1 knockout hermaphrodites displayed a shortened lifespan and elongated defecation intervals. Conclusions. The FOLT-1 protein function affects both the soma and the germline. folt-1(ok1460) hermaphrodites suffer severely diminished lifespan and germline defects that result in sterility. Germline defects associated with folate deficiency appear widespread in animals, being found in humans, mice, fruit flies, and here, nematodes. © 2010 Austin et al; licensee BioMed Central Ltd. | Source Title: | BMC Developmental Biology | URI: | https://scholarbank.nus.edu.sg/handle/10635/181665 | ISSN: | 1471213X | DOI: | 10.1186/1471-213X-10-46 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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