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https://doi.org/10.1186/1472-6882-13-150
Title: | Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don | Authors: | Dai, Z.-J Lu, W.-F Gao, J Kang, H.-F Ma, Y.-G Zhang, S.-Q Diao, Y Lin, S Wang, X.-J Wu, W.-Y |
Keywords: | 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide apigenin baicalein luteolin vasculotropin angiogenesis animal experiment antiangiogenic activity article cell migration cell proliferation cell viability chick embryo chorioallantois concentration (parameters) controlled study embryo enzyme linked immunosorbent assay in vitro study in vivo study nonhuman Scutellaria barbata umbilical vein endothelial cell Angiogenesis Inhibitors Animals Chick Embryo Chorioallantoic Membrane Flavonoids Human Umbilical Vein Endothelial Cells Plant Extracts Scutellaria Vascular Endothelial Growth Factor A |
Issue Date: | 2013 | Citation: | Dai, Z.-J, Lu, W.-F, Gao, J, Kang, H.-F, Ma, Y.-G, Zhang, S.-Q, Diao, Y, Lin, S, Wang, X.-J, Wu, W.-Y (2013). Anti-angiogenic effect of the total flavonoids in Scutellaria barbata D. Don. BMC Complementary and Alternative Medicine 13 : 150. ScholarBank@NUS Repository. https://doi.org/10.1186/1472-6882-13-150 | Rights: | Attribution 4.0 International | Abstract: | Background: Angiogenesis is closely related to the growth, invasion and metastasis of tumors, also considered as the key target of anticancer therapy. Scutellaria barbata D. Don (S. barbata), a traditional Chinese medicine, is being used to treat various diseases, including cancer. However, the antitumor molecular mechanism of S. barbata was still unclear. This study aimed to investigate the inhibitory effects of the total flavones in S. barbata (TF-SB) on angiogenesis.Methods: Human umbilical vein endothelial cells (HUVECs) were treated with various concentrations of TF-SB. Cell viability was examined using the MTT assay. The scratch assay was used to detect the migration of HUVECs after treatment with TF-SB. The ability of HUVECs to form network structures in vitro was demonstrated using the tube formation assay. The chick embryo chorioallantoic membrane assay was performed to detect the in vivo anti-angiogenic effect. The expression of VEGF was measured by the enzyme-linked immunosorbent.Results: Results showed that TF-SB inhibited the proliferation and migration of HUVECs in a dose- dependent manner. Simultaneously, TF-SB significantly suppressed HUVEC angiogenesis in vitro and in vivo. Furthermore, VEGF was downregulated in both HUVECs and MHCC97-H cells after TF-SB treatment.Conclusion: TF-SB could suppress the process of angiogenesis in vitro and in vivo. TF-SB potentially suppresses angiogenesis in HUVECs by regulating VEGF. These findings suggested that TF-SB may serve as a potent anti-angiogenic agent. © 2013 Dai et al.; licensee BioMed Central Ltd. | Source Title: | BMC Complementary and Alternative Medicine | URI: | https://scholarbank.nus.edu.sg/handle/10635/181566 | ISSN: | 14726882 | DOI: | 10.1186/1472-6882-13-150 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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