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https://doi.org/10.1186/s12864-015-2158-0
Title: | Identification of TTAGGG-binding proteins in Neurospora crassa, a fungus with vertebrate-like telomere repeats | Authors: | Casas-Vila, N Scheibe, M Freiwald, A Kappei, D Butter, F |
Keywords: | Gbp2 protein protein Myb Tay1 protein Tbf1 protein Tcg1 protein telomere binding protein unclassified drug zinc finger protein DNA binding protein fungal protein amino acid sequence Article controlled study genetic conservation Neurospora crassa nonhuman phylogenomics protein analysis protein DNA interaction protein domain proteomics quantitative analysis Saccharomyces cerevisiae Schizosaccharomyces pombe species comparison telomere animal conserved sequence genetics metabolism molecular evolution Neurospora crassa nucleotide motif nucleotide repeat nucleotide sequence proteomics telomere vertebrate Animals Base Sequence Conserved Sequence DNA-Binding Proteins Evolution, Molecular Fungal Proteins Neurospora crassa Nucleotide Motifs Proteomics Repetitive Sequences, Nucleic Acid Telomere Vertebrates |
Issue Date: | 2015 | Citation: | Casas-Vila, N, Scheibe, M, Freiwald, A, Kappei, D, Butter, F (2015). Identification of TTAGGG-binding proteins in Neurospora crassa, a fungus with vertebrate-like telomere repeats. BMC Genomics 16 (1) : 965. ScholarBank@NUS Repository. https://doi.org/10.1186/s12864-015-2158-0 | Rights: | Attribution 4.0 International | Abstract: | Background: To date, telomere research in fungi has mainly focused on Saccharomyces cerevisiae and Schizosaccharomyces pombe, despite the fact that both yeasts have degenerated telomeric repeats in contrast to the canonical TTAGGG motif found in vertebrates and also several other fungi. Results: Using label-free quantitative proteomics, we here investigate the telosome of Neurospora crassa, a fungus with canonical telomeric repeats. We show that at least six of the candidates detected in our screen are direct TTAGGG-repeat binding proteins. While three of the direct interactors (NCU03416 [ncTbf1], NCU01991 [ncTbf2] and NCU02182 [ncTay1]) feature the known myb/homeobox DNA interaction domain also found in the vertebrate telomeric factors, we additionally show that a zinc-finger protein (NCU07846) and two proteins without any annotated DNA-binding domain (NCU02644 and NCU05718) are also direct double-strand TTAGGG binders. We further find two single-strand binders (NCU02404 [ncGbp2] and NCU07735 [ncTcg1]). Conclusion: By quantitative label-free interactomics we identify TTAGGG-binding proteins in Neurospora crassa, suggesting candidates for telomeric factors that are supported by phylogenomic comparison with yeast species. Intriguingly, homologs in yeast species with degenerated telomeric repeats are also TTAGGG-binding proteins, e.g. in S. cerevisiae Tbf1 recognizes the TTAGGG motif found in its subtelomeres. However, there is also a subset of proteins that is not conserved. While a rudimentary core TTAGGG-recognition machinery may be conserved across yeast species, our data suggests Neurospora as an emerging model organism with unique features. © 2015 Casas-Vila et al. | Source Title: | BMC Genomics | URI: | https://scholarbank.nus.edu.sg/handle/10635/181420 | ISSN: | 14712164 | DOI: | 10.1186/s12864-015-2158-0 | Rights: | Attribution 4.0 International |
Appears in Collections: | Elements Staff Publications |
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