Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/17731
Title: Kinectin in protein synthesis and membrane dynamics
Authors: HENG KIANG, JUSTIN
Keywords: Kinectin, Endoplasmic Reticulum, Migration, Attachment
Issue Date: 29-Dec-2009
Source: HENG KIANG, JUSTIN (2009-12-29). Kinectin in protein synthesis and membrane dynamics. ScholarBank@NUS Repository.
Abstract: This study investigates the role of ER dynamics via kinectin, a membrane anchor linking ER to kinesin, on cellular functions. Over-expression of the binding domains of both kinectin (KNT+) and kinesin (KHC+) result in reduction to ER extension, cellular migration and attachment. ER dynamics' role in mediating cellular adhesion to ECM proteins was further probed. Fibronectin and collagen both induced increased cellular attachment whereas laminin did not. Disruption of the kinectin-kinesin interaction reduced cellular attachment on fibronectin but not collagen surfaces, suggesting that fibronectin functions in an ER dependant manner. ECM-coated beads were used as localized signals and fibronectin-coated beads appeared to significantly induce ER accumulation while neither collagen- nor laminin-coated beads managed significant improvements in ER accumulation.A model emerges whereby ER accumulation, mediated by the kinectin-kinesin interaction, to sites of new focal adhesion formation is required for proper cellular attachment and migration.
URI: http://scholarbank.nus.edu.sg/handle/10635/17731
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