Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/17638
Title: Differentiation and derivation of lineage-committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
Authors: TOH WEI SEONG
Keywords: human, embryonic stem cells, chondrogenic, cartilage, tissue engineering, regenerative medicine
Issue Date: 26-Feb-2010
Source: TOH WEI SEONG (2010-02-26). Differentiation and derivation of lineage-committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration. ScholarBank@NUS Repository.
Abstract: Articular cartilage repair is problematic due to its poor self-regenerative ability. This study explored the potential of human embryonic stem cells (hESCs) and its derivatives for cartilage tissue engineering and regeneration. However, major challenges impeding clinical application of hESCs included issues of tumorigenicity and functionality upon transplantation. Lineage-restricted chondrogenic cell lines were generated from hESCs and demonstrated to be expandable, non-tumorigenic, functional, and stable as a cell source for cartilage tissue engineering. When implanted into critical-sized osteochondral defects in rats, hESC-derived engineered cartilage (HCCEC) demonstrated an orderly spatial-temporal remodeling process over 12 weeks into osteochondral tissue, marked by development of characteristic architectural features including a hyaline-like neocartilage layer with complete integration with the adjacent host cartilage and a regenerated subchondral bone. The transplanted hESC-derived chondrogenic cells maintained long-term viability with no evidence of tumorigenicity, henceforth providing a safe, highly-efficient and practical strategy of applying hESCs for cartilage regeneration.
URI: http://scholarbank.nus.edu.sg/handle/10635/17638
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
TohWS-PhDThesis-2010.pdf5.1 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

370
checked on Dec 11, 2017

Download(s)

145
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.