Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/17637
Title: Group 2 allergens from dust mite: Epitope mapping and functional characterization of Der p 2, and identification of a paralogue of Der f 2
Authors: KAVITA D/O REGINALD
Keywords: Allergy, dust mite, group 2 allergen, hypoallergen, epitope mapping, paralogue
Issue Date: 12-Jan-2007
Source: KAVITA D/O REGINALD (2007-01-12). Group 2 allergens from dust mite: Epitope mapping and functional characterization of Der p 2, and identification of a paralogue of Der f 2. ScholarBank@NUS Repository.
Abstract: Group 2 allergens from dust mites cause allergies in >60% of dust mite sensitized individuals. Der p 2 was the most allergenic of the eight group 2 allergens tested in individuals from two populations (Singaporean and Italian). Following this observation, the IgE epitopes of Der p 2 was characterized in the same populations using single alanine site directed mutants of Der p 2. Three mutants (E25A, E102A and K96A) showing consistent reduced IgE reactions compared to wild type Der p 2 were then evaluated for their efficacy as hypoallergen vaccine candidates. Mutants E102A and K96A (resulting in an unfolded protein) were potential hypoallergen vaccine candidates as they demonstrated reduced IgE reaction, no skin prick reactivity, ability to elicit blocking IgG antibodies and stimulation of T cell proliferation. In addition, a new allergen, Der f 22 was isolated and characterized. This allergen has low amino acid sequence identity to Der f 2 (a group 2 allergen) but shared functional similarities, and was therefore hypothesized to be a putative paralogue of Der f 2. The biochemical function(s) of group 2 allergens are unknown to date. To aid the elucidation of its function, the ligand of Der p 2 was characterized. Der p 2 was observed to bind preferentially to cholesterol, amoung structurally similar sterols. Cholesterol is likely to bind within the hydrophobic cavity of Der p 2 with multiple binding sites.
URI: http://scholarbank.nus.edu.sg/handle/10635/17637
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