Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/17596
Title: Molecular characterization and developmental analysis of the TGF Beta 3 gene in Zebrafish
Authors: CHEAH SIEW HONG
Keywords: TGFB3, zebrafish, neural crest, pharyngeal arch, notochord, heart
Issue Date: 25-Apr-2007
Source: CHEAH SIEW HONG (2007-04-25). Molecular characterization and developmental analysis of the TGF Beta 3 gene in Zebrafish. ScholarBank@NUS Repository.
Abstract: TGFB3, a member of the transforming growth factor beta family, regulates a plethora of biological processes and is involved in mammalian pulmonary and craniofacial development. I have characterized the full-length cDNA and genomic organization of zebrafish tgfb3, and observed a high degree of nucleotide and amino acid conservation with other species, including a complete conservation of the cysteine knot structure that facilitates protein-protein interaction. Zebrafish tgfb3 transcripts were first detected in the notochord, followed by expression in the developing pharyngeal arch and neurocranial cartilage, lens and heart, and pectoral fins. Perturbation of tgfb3 gene revealed possible roles in head skeletogenesis and cardiac development. Both knockdown and overexpression studies offer supportive evidence that the effects of tgfb3 during embryonic development are dosage dependent and tissue specific. Despite TGFB3 is extensively studied in other vertebrate models, we have found novel functions of this gene in cardiac development that are yet to be discovered in other vertebrate models.
URI: http://scholarbank.nus.edu.sg/handle/10635/17596
Appears in Collections:Ph.D Theses (Open)

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