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Title: Regulation of subcellular localization and functions of RGK proteins by 14-3-3 and calmodulin
Keywords: RGK proteins, nuclear transport, calmodulin, 14-3-3
Issue Date: 9-May-2007
Citation: RAMASUBBU NARAYANAN MAHALAKSHMI (2007-05-09). Regulation of subcellular localization and functions of RGK proteins by 14-3-3 and calmodulin. ScholarBank@NUS Repository.
Abstract: Kir/Gem, Rad, Rem and Rem2 are members of RGK family of Ras related GTPases which are implicated in important functions such as the regulation of voltage gated calcium channels (VDCCs) and cell shape remodeling. RGK proteins interact with 14-3-3 and calmodulin, but their role on RGK protein function is poorly understood. In the current study, we show that two regulatory proteins alter the subcellular localization of RGK proteins through nucleocytoplasmic transport and also modulate the cell shape changes induced by RGK proteins. Our results show that RGK proteins down regulate calcium channel activity by interfering with the cell surface expression of the alpha subunit, through the sequestration of beta-3 subunit. Further we found that calmodulin but not 14-3-3 modulates the downregulation of calcium channel activity by RGK proteins. Furthermore, we identified three conserved nuclear localization signals in RGK proteins and identified the importins needed for the nuclear transport of the small GTPases. Thus our investigations reveal that the functions of RGK family of Ras related small GTPases are subjected to multiple regulatory mechanisms.
Appears in Collections:Ph.D Theses (Open)

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