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Title: The role of heat shock protein 20 in the pathogenesis of Helicobacter pylori infections
Keywords: HSP20; Helicobacter pylori
Issue Date: 20-Apr-2007
Source: GOH YUN SHAN (2007-04-20). The role of heat shock protein 20 in the pathogenesis of Helicobacter pylori infections. ScholarBank@NUS Repository.
Abstract: Heat Shock Protein (HSP20), a surface localized protein of Helicobacter pylori, was shown to co-immunoprecipitate with CagA (cytotoxin-associated gene A), a well-studied pathogenic factor. Interestingly, CagA being a cytoplasmic protein has been reported to be surface exposed before being translocated across Type IV secretion system into the host cells during H. pylori infection. Transmission electron microscopy findings show that upon host contact there is a co-localization of HSP20 with CagA in the cell cytoplasm. Western blotting study shows that in the absence of HSP20 the efficiency of CagA translocation was evidently reduced. In addition HSP20 assists in CagA-mediated assembly of tight junctional ZO-1 to extra-junctional bacterial attachment sites. This redistribution was shown to have a causal relationship with disruption of paracellular barrier function of tight junction complexes. The level of leakage decreased considerably in the absence of HSP20. Taken together, the role of HSP20 as a chaperone to CagA in facilitating H. pylori infections cannot be undermined.
Appears in Collections:Master's Theses (Open)

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