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https://doi.org/10.1038/s41598-017-11519-z
Title: | An embryonic system to assess direct and indirect Wnt transcriptional targets | Authors: | Suresh, J Harmston, N Lim, K.K Kaur, P Jin, H.J Lusk, J.B Petretto, E Tolwinski, N.S |
Keywords: | protein binding transcriptome Wnt protein animal apoptosis biology Drosophila embryology gene expression profiling gene expression regulation gene ontology metabolism mutation phenotype procedures transcription initiation Wnt signaling Animals Apoptosis Computational Biology Drosophila Gene Expression Profiling Gene Expression Regulation, Developmental Gene Ontology Mutation Phenotype Protein Binding Transcriptional Activation Transcriptome Wnt Proteins Wnt Signaling Pathway |
Issue Date: | 2017 | Citation: | Suresh, J, Harmston, N, Lim, K.K, Kaur, P, Jin, H.J, Lusk, J.B, Petretto, E, Tolwinski, N.S (2017). An embryonic system to assess direct and indirect Wnt transcriptional targets. Scientific Reports 7 (1) : 11092. ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-017-11519-z | Abstract: | During animal development, complex signals determine and organize a vast number of tissues using a very small number of signal transduction pathways. These developmental signaling pathways determine cell fates through a coordinated transcriptional response that remains poorly understood. The Wnt pathway is involved in a variety of these cellular functions, and its signals are transmitted in part through a ?-catenin/TCF transcriptional complex. Here we report an in vivo Drosophila assay that can be used to distinguish between activation, de-repression and repression of transcriptional responses, separating upstream and downstream pathway activation and canonical/non-canonical Wnt signals in embryos. We find specific sets of genes downstream of both ?-catenin and TCF with an additional group of genes regulated by Wnt, while the non-canonical Wnt4 regulates a separate cohort of genes. We correlate transcriptional changes with phenotypic outcomes of cell differentiation and embryo size, showing our model can be used to characterize developmental signaling compartmentalization in vivo. © 2017 The Author(s). | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/175168 | ISSN: | 20452322 | DOI: | 10.1038/s41598-017-11519-z |
Appears in Collections: | Elements Staff Publications |
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