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Title: Regulation of chondrocyte differentiation: potential involvement of Wnt/ß-catenin signaling
Keywords: cartilage, wnt, chondrocyte, differentiation, hyaline, growth factors, curcumin, ß-catenin
Issue Date: 4-Jan-2010
Citation: AFIZAH BINTE MOHD HASSAN (2010-01-04). Regulation of chondrocyte differentiation: potential involvement of Wnt/ß-catenin signaling. ScholarBank@NUS Repository.
Abstract: This study is aimed at investigating the inhibition of chondrocyte phenotype loss during in vitro expansion through antagonizing the Wnt/ß-catenin signaling pathway, to generate cartilage of a higher hyaline quality for ACI repair. Chondrocytes were cultured in monolayer and exposed to a cocktail of growth factors (TFP) and the Wnt/ß-catenin antagonist, curcumin. Redifferentiation ability of the cells that were exposed to different expansion conditions was then tested through pellet culture. Hyaline and fibro- cartilage markers were analysed to assess the type of cartilage formed. Our results suggest an association between ß-catenin expression and subsequent redifferentiated tissue quality. Addition of curcumin in conjunction with growth factor cocktail during monolayer expansion led to the formation of purer hyaline cartilage. This is clinically significant since current cartilage repair techniques were found to produce hyaline cartilage only after a few years. This could translate to a faster healing for patients suffering from cartilage damage.
Appears in Collections:Master's Theses (Open)

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