Please use this identifier to cite or link to this item:
https://doi.org/10.1021/acsami.8b19391
Title: | Visualize Embryogenesis and Cell Fate Using Fluorescent Probes with Aggregation-Induced Emission | Authors: | Hu, Fang Manghnani, Purnima Naresh KENRY FENG GUANGXUE Wu, Wenbo Teh, Cathleen LIU BIN |
Keywords: | Science & Technology Technology Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science aggregation-induced emission small molecule bright signals embryonic cell zebrafish lineage tracing cell entrapped INTRACELLULAR INJECTION ANTIBACTERIAL ACTIVITY LINEAGE ANALYSIS LUMINOGENS |
Issue Date: | 30-Jan-2019 | Publisher: | American Chemical Society | Citation: | Hu, Fang, Manghnani, Purnima Naresh, KENRY, FENG GUANGXUE, Wu, Wenbo, Teh, Cathleen, LIU BIN (2019-01-30). Visualize Embryogenesis and Cell Fate Using Fluorescent Probes with Aggregation-Induced Emission. ACS Applied Materials and Interfaces 11 (4) : 3737-3744. ScholarBank@NUS Repository. https://doi.org/10.1021/acsami.8b19391 | Abstract: | Horseradish peroxidase (HRP) and fluorogen-dextran conjugate are tracers extensively used for injection-based lineage tracing. However, HRP is sensitive to proteolytic digestion, whereas the high-molecular-weight dextran may have antigenicity. Small molecular tracers can overcome these problems, but they usually diffuse from labeled cells, causing inaccurate information. Herein, we developed a small-molecular-weight fluorogen with aggregation-induced emission (AIEgen) for embryonic cell tracing with strong signals against tracer dilution caused by cell division. Once injected into the ancestor cells, the AIEgen can be entrapped in the cells without leakage because of the two hydrophilic and neutral arms. Consequently, it can specifically trace the progenies of the treated ancestor cells. More importantly, the operating concentration of AIEgen can be much higher than that of fluorogens with aggregation-caused quenching, which provides bright signals in daughter cells during embryonic cell tracing, thus overcoming the problem of fast signal degradation typically encountered with the use of traditional cell tracers. | Source Title: | ACS Applied Materials and Interfaces | URI: | https://scholarbank.nus.edu.sg/handle/10635/170709 | ISSN: | 1944-8244 1944-8252 |
DOI: | 10.1021/acsami.8b19391 |
Appears in Collections: | Staff Publications Elements |
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visualize.pdf | Accepted version | 1.29 MB | Adobe PDF | OPEN | Post-print | View/Download |
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