Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/17049
Title: Signal modulation of type III secretion system in pseudomonas aeruginosa
Authors: ZHOU LIAN
Keywords: Pseudomonas aeruginosa, T3SS, signal modulation, polyamines
Issue Date: 2-Jul-2009
Source: ZHOU LIAN (2009-07-02). Signal modulation of type III secretion system in pseudomonas aeruginosa. ScholarBank@NUS Repository.
Abstract: Type III secretion system (T3SS) is a key virulence determinant in opportunistic human pathogen Pseudomonas aeruginosa. Expression of T3SS genes is under the tight control of its master regulator ExsA and other regulatory proteins encoded by the exsCEBA operon. In this study, I set to explore the potential signaling mechanisms which modulate the expression of exsCEBA. By screening a transposon mutant library of P. aeruginosa, I found that mutation of spuDEFGH, which encodes a major spermidine uptake system, abolished the expression of most T3SS genes, including the exsCEBA operon, under low calcium inducing condition. The mutation also resulted in attenuated cytotoxicity. Consistently, exogenous addition of spermidine to the wild type strain PAO1 enhanced the expression of exsCEBA and induced the secretion of the effector ExoS, demonstrating that a spermidine transporter-dependent signaling pathway is involved in T3SS regulation. By deletion analysis of the genes up-regulated in spu tr
URI: http://scholarbank.nus.edu.sg/handle/10635/17049
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
ZHOU lian HT031222Y - SIGNAL MODULATION OF TYPE III SECRETION SYSTEM IN PSEUDOMONAS AERUGINOSA.pdf1.88 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

229
checked on Dec 11, 2017

Download(s)

330
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.