Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/17009
Title: Molecular and cellular studies of shoot morphogenesis In vitro in relation to polyamines and hydrogen peroxide
Authors: LIM TZE SOO
Keywords: flavin-amine oxidase, shoot organogenesis, polyamines, hydrogen peroxide, mustard, Arabidopsis
Issue Date: 7-Sep-2006
Source: LIM TZE SOO (2006-09-07). Molecular and cellular studies of shoot morphogenesis In vitro in relation to polyamines and hydrogen peroxide. ScholarBank@NUS Repository.
Abstract: In recent years, increasing data have indicated that polyamines (PAs) are involved in the shoot organogenesis process in vitro, and that PA action may be mediated through hydrogen peroxide (H2O2), a product of PA oxidation. Flavin amine oxidases (FAO) are involved in the catabolism of PAs that generate H2O2, which can also be produced by superoxide dismutase (SOD). As a part of the study to investigate whether shoot organogenesis in vitro is associated with H2O2 through PA catabolism, FAO genes were cloned and characterized from mustard and Arabidopsis. Transgenic Arabidopsis plants with overexpression and downregulation of FAO and SOD cDNAs were also created and assessed for shoot regeneration capacities. The results obtained showed that shoot regeneration in vitro was associated with increased PA levels, rather than modulation of H2O2 levels. Moreover, transgenic FAO plants neither conferred stress tolerance nor exhibited abnormal phenotypes.
URI: http://scholarbank.nus.edu.sg/handle/10635/17009
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Title page.pdf52.39 kBAdobe PDF

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Table of contents.pdf162.18 kBAdobe PDF

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C1-General Introduction.pdf76.44 kBAdobe PDF

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C2-Literature Review.pdf265.62 kBAdobe PDF

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C3-Materials and Methods.pdf285.46 kBAdobe PDF

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C4-C6-Results.pdf3.29 MBAdobe PDF

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C7-General discussion and conclusion.pdf83.72 kBAdobe PDF

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References.pdf203.17 kBAdobe PDF

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