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Title: | Molecular and cellular studies of shoot morphogenesis In vitro in relation to polyamines and hydrogen peroxide | Authors: | LIM TZE SOO | Keywords: | flavin-amine oxidase, shoot organogenesis, polyamines, hydrogen peroxide, mustard, Arabidopsis | Issue Date: | 7-Sep-2006 | Citation: | LIM TZE SOO (2006-09-07). Molecular and cellular studies of shoot morphogenesis In vitro in relation to polyamines and hydrogen peroxide. ScholarBank@NUS Repository. | Abstract: | In recent years, increasing data have indicated that polyamines (PAs) are involved in the shoot organogenesis process in vitro, and that PA action may be mediated through hydrogen peroxide (H2O2), a product of PA oxidation. Flavin amine oxidases (FAO) are involved in the catabolism of PAs that generate H2O2, which can also be produced by superoxide dismutase (SOD). As a part of the study to investigate whether shoot organogenesis in vitro is associated with H2O2 through PA catabolism, FAO genes were cloned and characterized from mustard and Arabidopsis. Transgenic Arabidopsis plants with overexpression and downregulation of FAO and SOD cDNAs were also created and assessed for shoot regeneration capacities. The results obtained showed that shoot regeneration in vitro was associated with increased PA levels, rather than modulation of H2O2 levels. Moreover, transgenic FAO plants neither conferred stress tolerance nor exhibited abnormal phenotypes. | URI: | http://scholarbank.nus.edu.sg/handle/10635/17009 |
Appears in Collections: | Ph.D Theses (Open) |
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Cover.pdf | 32.71 kB | Adobe PDF | OPEN | None | View/Download | |
Title page.pdf | 52.39 kB | Adobe PDF | OPEN | None | View/Download | |
Table of contents.pdf | 162.18 kB | Adobe PDF | OPEN | None | View/Download | |
C1-General Introduction.pdf | 76.44 kB | Adobe PDF | OPEN | None | View/Download | |
C2-Literature Review.pdf | 265.62 kB | Adobe PDF | OPEN | None | View/Download | |
C3-Materials and Methods.pdf | 285.46 kB | Adobe PDF | OPEN | None | View/Download | |
C4-C6-Results.pdf | 3.29 MB | Adobe PDF | OPEN | None | View/Download | |
C7-General discussion and conclusion.pdf | 83.72 kB | Adobe PDF | OPEN | None | View/Download | |
References.pdf | 203.17 kB | Adobe PDF | OPEN | None | View/Download |
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