Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16948
Title: Characterisation of osteogenic cells differentiated from human embryonic stem cells in comparison with mesenchymal stem cells
Authors: YE CHAOPENG
Keywords: human embryonic stem cell, MSC, osteogenic differentiation
Issue Date: 19-Oct-2005
Source: YE CHAOPENG (2005-10-19). Characterisation of osteogenic cells differentiated from human embryonic stem cells in comparison with mesenchymal stem cells. ScholarBank@NUS Repository.
Abstract: Osteogenic cells differentiated from both human embryonic stem cells (hES) and mesenchymal stem cells (MSC) hold much promise in both clinic application and basic science research. Previously, only two studies reported that osteogenic differentiation of hES was achieved. Although osteogenic differentiation from ex vivo cultured MSCs was readily achieved, there were few well-defined and efficient protocols to promote the osteogenesis.First, this study attempted a new approach to achieve osteogenesis within intact human embryoid bodies in osteogenesis-promoting medium. Secondly this study investigated whether a combination of chemical stimuli and conditioned media from heat shock treated human fetal osteoblast cell line (hFOB) had any synergistic effect on the osteogenesis of MSC.Osteogenic differentiation within intact human embryoid bodies result in a marked increase in osteocalcin secretion, and formation of nodule-like structures where osteogenic cells are detected. Culture media conditioned by heat-shocked fetal osteoblast cell line significantly enhances the osteogenesis of MSC.
URI: http://scholarbank.nus.edu.sg/handle/10635/16948
Appears in Collections:Master's Theses (Open)

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Chapter I literature review.pdf199.22 kBAdobe PDF

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Chapter II Materials And Methods.pdf331.74 kBAdobe PDF

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Chapter III result and Chapter IV discussion.pdf1.02 MBAdobe PDF

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CHAPTER V Conclusion.pdf12.06 kBAdobe PDF

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Biblography.pdf174.05 kBAdobe PDF

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Publications.pdf10.76 kBAdobe PDF

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