Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16808
Title: The integrator subunits function in hematopoiesis by modulating SMAD/BMP signaling
Authors: TAO SHIJIE
Keywords: Integrator subunits, smad5, BMP, hematopoiesis, RNA processing, red blood cell differentiation
Issue Date: 3-Jul-2009
Source: TAO SHIJIE (2009-07-03). The integrator subunits function in hematopoiesis by modulating SMAD/BMP signaling. ScholarBank@NUS Repository.
Abstract: The Integrator complex proteins are known to directly interact with RNA polymerase II to mediate 3b end processing of U1 and U2 snRNAs. We have identified several subunits of the Integrator complex in zebrafish. Knock-down of the Integrator subunit 5 (Ints5) in zebrafish embryos causes aberrant splicing of smad1 and smad5 RNA, and reduced expression of the hematopoietic genes. Blood smears from ints5 morphant embryos show arrested red blood cell differentiation. Interestingly, targeting other Integrator subunits also leads to defects in smad5 RNA splicing and arrested hematopoiesis, suggesting that the Int proteins function as a complex to regulate the BMP (Bone Morphogenetic Protein) pathway during hematopoiesis. Our work establishes a link between the RNA processing machinery and the downstream effectors of BMP signaling, and reveals a new group of proteins that regulate the switch from primitive hematopoietic stem cell identity and blood cell differentiation by modulating Smad function.
URI: http://scholarbank.nus.edu.sg/handle/10635/16808
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
TaoSJ.pdf25.66 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

199
checked on Dec 11, 2017

Download(s)

97
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.