Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16802
Title: Regulation of antigen presentation in dendritic cells
Authors: HUANG DACHUAN
Keywords: antigen presentation,dendritic cells,DC-CASPIC,CD74,Nitric oxide synthase,caspase
Issue Date: 22-Aug-2008
Source: HUANG DACHUAN (2008-08-22). Regulation of antigen presentation in dendritic cells. ScholarBank@NUS Repository.
Abstract: In this project, we identified and described the function of a novel isoform of CIITA, DC-CASPIC in mitochondria in dendritic cells (DCs). DC-CASPIC inhibits the caspase-dependent degradation of NOS2 and induces NO synthesis in maturing DCs. Furthermore, DCs over-expressing DC-CASPIC enhance MHC class II, CD80 and CD86 cell surface expression and stimulate T cell proliferation. Taken together, our results strongly suggest that DC-CASPIC is one of the key molecules that regulate NO synthesis and antigen presentation during maturation of DCs.We also reported that in immature DCs, the NO donor and the over-expression of either NOS2 or NOS3 alone could induce the cell surface expression of MHC class II, CD80 and CD86 and enhance T cell proliferation in vitro. Interestingly, NOS2 interacted with CD74, and the degradation of CD74 by caspases in immature DCs was inhibited upon treatment with the NO donor.
URI: http://scholarbank.nus.edu.sg/handle/10635/16802
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