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Title: The role of Kruppel-like factors in embryonic stem cells
Keywords: embryonic stem cell, self-renewal, pluripotency, reprogramming, gene regulation
Issue Date: 30-Jun-2009
Citation: JIANG JIANMING (2009-06-30). The role of Kruppel-like factors in embryonic stem cells. ScholarBank@NUS Repository.
Abstract: Embryonic stem (ES) cells are able to self-renew and maintain capacity in differentiation. These properties require transcription factors that specify the gene expression program of ES cells. Highly differentiated state of somatic cells can be reverse back to pluripotent state by defined transcription factors. Klf4 is one of the required transcription factors in conjunction with Oct4, Sox2 and c-Myc. Here, we show that Klf2, Klf4 and Klf5 are required for the self-renewal of ES cells. Simultaneous depletion of these three Klfs leads to cell differentiation. ChIP-on-chip reveals that these Klf proteins share many common targets, suggesting a functional relationship between these factors. Expression analysis after triple knockdown of the Klfs shows that they regulate key pluripotency genes, such as Nanog. Klf4 can be substituted by Klf2 or Klf5 in inducing reprogrammed cells. Together, our study sheds new insights into how the Klfs integrate into transcriptional network to maintain pluripotent state.
Appears in Collections:Ph.D Theses (Open)

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