Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16529
Title: Can DNA sequences help with sorting biodiversity samples?
Authors: LIM SHIMIN GWYNNE
Keywords: species richness estimation, DNA barcoding, cryptic species, widespread species, Sepsidae, Corethrellidae
Issue Date: 21-Aug-2009
Source: LIM SHIMIN GWYNNE (2009-08-21). Can DNA sequences help with sorting biodiversity samples?. ScholarBank@NUS Repository.
Abstract: Corethrellid fly specimens exemplify the daunting task of processing tropical arthropods, as the large numbers of specimens and lack of described species pose a major challenge for non-specialists. To further complicate the matter, individuals of common morphotypes overwhelmingly dominate these samples, meaning that much time and effort must be expended to discover rare types. One potential way to expedite this process is to use a series of quick DNA extraction-cum-amplification techniques to pre-sort specimens based on DNA sequences. Furthermore, morphological details are usually well retained, allowing future taxonomic work. This is an improvement from parataxonomic sorting, which cannot be combined across studies and may have higher levels of error. I further demonstrate the facility of these techniques and their application in cosmopolitan family Sepsidae, a taxonomically well understood family of saprophagous flies. Using molecular information extracted from the specimens, I estimate species richness and re-evaluate the boundaries of widespread species.
URI: http://scholarbank.nus.edu.sg/handle/10635/16529
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