Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/16429
Title: Surface functionalization of nano-magnetic particle with beta cyclodextrin and its use in bio-molecule refolding process
Authors: NGUYEN THI KHANH THUYEN
Keywords: nano magnetic particles, beta cyclodextrin, lysozyme, refolding, denatured protein, aminopropyl
Issue Date: 18-Jun-2007
Source: NGUYEN THI KHANH THUYEN (2007-06-18). Surface functionalization of nano-magnetic particle with beta cyclodextrin and its use in bio-molecule refolding process. ScholarBank@NUS Repository.
Abstract: In recent years, the using of nano-sized magnetic particles in the field of bioengineering is gaining more interest. Due to their size, magnetic nanoparticles providelarge specific surface areas and moreover, the magnetic properties of these particles offer an excellent tool to handle particles in suspension by using magnetic field. However, to apply in the fields of bioengineering, surface modification of magnetic nanoparticles must be carried out with inorganic or organic materials. The present work deals with surface functionalization of magnetic nanoparticles with I?-cyclodextrin and its application onrefolding of denatured biomolecules.First, magnetic nanoparticles were prepared by co-precipitation of Fe2+ and Fe3+ saltsat a 1:2 molar ratio with ammonium hydroxide (NH4OH). The magnetic nanoparticleswere then coated with aminopropyl silane with amine (a??NH2) groups. The amine (a??NH2)groups were attached to the surface of magnetic nanoparticles through condensation ofaminopropyltriethoxysilane (APTS) reaction. Finally, I?-cyclodextrin bonded nano-sizedmagnetic particles were prepared through reaction between mono-tosyl-I?-cyclodextrin(Ts-I?-CD) and aminopropyl silane coated magnetic particles. The synthesis of monotosyl-I?-cyclodextrin was carried out by reacting I?-cyclodextrin with a solution of ptoluene-sulfonyl chloride in dry pyridine.The structural information of magnetic nanoparticles coated by aminosilane and I?-cyclodextrin bonded magnetic nanoparticles was characterized and confirmed usingTransmission Electronic Microscopy (TEM), X-ray Photoelectron Spectroscopy (XPS),X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Fourier Transform Raman (FT-Raman) and elemental analysis. The bare magnetic particles and the I?-cyclodextrin bonded magnetic particles were found to be in nano-sized and I?-cyclodextrin was attached on the surface of magnetic nanoparticles.In the present work, I?-cyclodextrin bonded magnetic nanoparticles have been successfully synthesized and applied in refolding of denatured lysozyme process. Theseparticles have significantly improved the refolding yield compared with bare magneticnanoparticles and magnetic nanoparticles coated with aminosilane. Moreover, undercertain experimental conditions, 71% refolding yield has been obtained for the denatured lysozyme at a concentration of 0.05mg/ml.
URI: http://scholarbank.nus.edu.sg/handle/10635/16429
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